This assay is useful for evaluation of men with signs and symptoms of possible Hypogonadism like loss of libido, erectile dysfunction, gynecomastia & infertility. It is also useful in evaluation of boys with delayed or precocious puberty. The assay can be used to monitor anti- androgen therapy as in prostate cancer, precocious puberty & male to female transgender disorders. It helps to evaluate infants with ambiguous genitalia or virilization. The assay can serve as an adjunct in the diagnosis of androgen secreting tumors.